Haploid induction via unpollinated ovule culture in Gerbera hybrida
نویسندگان
چکیده
منابع مشابه
In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus
In order to increase the yield of haploid cucumber plants obtained from in vitro culture of unpollinated ovules, anther and microspore, we have studied several factors: the nature and concentration of plant growth regulators, the genotypes, the flower bud lengths, and the stages of microspore development. Among the different growth regulators tested, thidiazuron (TDZ) was the best for gynogenes...
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Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...
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Chrysanthemum is one of important ornamental species in the world. Its highly heterozygous state complicates molecular analysis, so it is of interest to derive haploid forms. A total of 2579 non-fertilized chrysanthemum ovules pollinated by Argyranthemum frutescens were cultured in vitro to isolate haploid progeny. One single regenerant emerged from each of three of the 105 calli produced. Chro...
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Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-58552-z